Human CUL3 Recombinant Protein (N-His) (HC660012)

Name: Human CUL3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC660012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HC660012
Description	Recombinant Human CUL3 Protein, N-His (HC660012) expressed in E. coli, spanning Met1-Ala768. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13618
Protein length	Met1-Ala768
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	92.15 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cullin-3, KIAA0617, CUL-3, CUL3
Background	Cullin-3 (CUL3) is a ~88 kDa protein. Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. BCR complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component. 1. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346 2. Jin, L. et al. (2012) Nature 482, 495-500. PMID: 22358839 3. McGourty, CA. et al. (2016) Cell 167, 525-538.e14. PMID: 27716508 4. Ohta, A. et al. (2013) The Biochemical journal 451, 111-22. PMID: 23387299 5. Wakabayashi, M. et al. (2013) Cell reports 3, 858-68. PMID: 23453970 6. Shibata, S. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 7838-43. PMID: 23576762 7. Lee, YR. et al. (2010) The EMBO journal 29, 1748-61. PMID: 20389280 8. Lin, MY. et al. (2011) The Journal of cell biology 193, 985-94. PMID: 21670212 9. Yuan, WC. et al. (2011) Cancer cell 20, 214-28. PMID: 21840486 10. Yuan, WC. et al. (2014) Molecular cell 54, 586-600. PMID: 24768539
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Human CUL3 Recombinant Protein (N-His)

References

Datasheet

Human CUL3 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human CUL3 Recombinant Protein (N-His) (HC660012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us