Human CYP2C8 Recombinant Protein (N-His) (HY115012)

Name: Human CYP2C8 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY115012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY115012
Description	Recombinant Human CYP2C8 Protein, N-His (HY115012) expressed in E. coli, spanning Gly40-Val490. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P10632
Protein length	Gly40-Val490
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	53.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CYP2C8, Cytochrome P450 MP-12, Cytochrome P450 IIC2, Cytochrome P450 MP-20, Cytochrome P450 2C8, CYPIIC8, S-mephenytoin 4-hydroxylase, Cytochrome P450 form 1
Background	Cytochrome P450 2C8 (CYP2C8) is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Primarily catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) with a preference for the last double bond. Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes all trans-retinoic acid toward its 4-hydroxylated form. 1. Marill, J. et al. (2000) Molecular pharmacology 58, 1341-8. PMID: 11093772 2. Lee, AJ. et al. (2003) Cancer research 63, 6532-6. PMID: 14559847 3. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564 4. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576 5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CYP2C8 Protein, N-His

References

HY115012-SDS-PAGE-1.jpg

Datasheet

Human CYP2C8 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human CYP2C8 Recombinant Protein (N-His) (HY115012)

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