Human CYP2C9 Recombinant Protein (N-His) (HB549012)

Name: Human CYP2C9 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB549012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB549012
Description	Recombinant Human CYP2C9 Protein, N-His (HB549012) expressed in E. coli, spanning Gly40-Val490. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P11712
Protein length	Gly40-Val490
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	53.77 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cytochrome P450 2C9; 1.14.14.1; (R)-limonene 6-monooxygenase; 1.14.14.53; (S)-limonene 6-monooxygenase; 1.14.14.51; (S)-limonene 7-monooxygenase; 1.14.14.52; CYPIIC9; Cholesterol 25-hydroxylase; Cytochrome P-450MP; Cytochrome P450 MP-4; Cytochrome P450 MP-8; Cytochrome P450 PB-1; S-mephenytoin 4-hydroxylase; CYP2C9; CYP2C10
Background	Cytochrome P450 2C9 is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. 1. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317 2. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564 3. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576 4. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599 5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697 6. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160 7. Bylund, J. et al. (1998) Analytical biochemistry 265, 55-68. PMID: 9866708 8. Miyazawa, M. et al. (2002) Drug metabolism and disposition: the biological fate of chemicals 30, 602-7. PMID: 11950794
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human CYP2C9 Protein, N-His

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Datasheet

Human CYP2C9 Recombinant Protein (N-His).pdf

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Formula

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C₁ × V₁ = C₂ × V₂

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Human CYP2C9 Recombinant Protein (N-His) (HB549012)

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