Human CYP2R1 Recombinant Protein (N-His) (HD360012)

Name: Human CYP2R1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD360012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD360012
Description	Recombinant Human CYP2R1 Protein, N-His (HD360012) expressed in E. coli, spanning Met37-Arg501. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6VVX0
Protein length	Met37-Arg501
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	55.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CYP2R1, Cytochrome P450 2R1, EC:1.14.14.24, Vitamin D 25-hydroxylase
Background	Vitamin D 25-hydroxylase is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). 1. Cheng, JB. et al. (2003) The Journal of biological chemistry 278, 38084-93. PMID: 12867411 2. Shinkyo, R. et al. (2004) Biochemical and biophysical research communications 324, 451-7. PMID: 15465040 3. Strushkevich, N. et al. (2008) Journal of molecular biology 380, 95-106. PMID: 18511070
Note	For research use only

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Datasheet

Human CYP2R1 Recombinant Protein (N-His).pdf

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Human CYP2R1 Recombinant Protein (N-His) (HD360012)

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