

| Catalog No. | HD894012 |
|---|---|
| Description |
Recombinant Human CYP4Z1 Protein, C-His (HD894012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | Q86W10 |
| Protein length | Arg31-Cys505 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | CYP4Z1, CYPIVZ1, Cytochrome P450 4Z1, EC:1.14.14.1, EC:1.14.14.130, Laurate 7-monooxygenase |
| Background | Cytochrome P450 4Z1 is a ~59 kDa protein. A cytochrome P450 monooxygenase that catalyzes the in-chain oxidation of fatty acids. Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates lauric and myristic acids predominantly at the omega-4 and omega-2 positions, respectively. Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Displays an absolute stereoselectivity in the epoxidation of arachidonic acid producing the 14(S),15(R)-epoxyeicosatrienoic acid (EET) enantiomer. 1. Zöllner, A. et al. (2009) Biological chemistry 390, 313-7. PMID: 19090726 2. McDonald, MG. et al. (2017) Drug metabolism and disposition: the biological fate of chemicals 45, 1364-1371. PMID: 29018033 |
| Note | For research use only |
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