Human CYP7B1 Recombinant Protein (N-His) (HD943012)

Name: Human CYP7B1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD943012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD943012
Description	Recombinant Human CYP7B1 Protein, N-His (HD943012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	O75881
Protein length	Gly57-Ser506
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	24-hydroxycholesterol 7-alpha-hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 3-hydroxysteroid 7-alpha hydroxylase, CYP7B1, Cytochrome P450 7B1, EC:1.14.14.26, EC:1.14.14.29, Oxysterol 7-alpha-hydroxylase
Background	Cytochrome P450 7B1 is a ~58 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. 1. Wu, Z. et al. (1999) Journal of lipid research 40, 2195-203. PMID: 10588945 2. Yantsevich, AV. et al. (2014) The FEBS journal 281, 1700-13. PMID: 24491228 3. Setchell, KD. et al. (1998) The Journal of clinical investigation 102, 1690-703. PMID: 9802883
Note	For research use only

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Datasheet

Human CYP7B1 Recombinant Protein (N-His).pdf

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Human CYP7B1 Recombinant Protein (N-His) (HD943012)

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