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Human DDA1 Recombinant Protein (N-GST & C-His) (HP866022)

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Overview
Catalog No.HP866022
Description
Recombinant Human DDA1 Protein, N-GST & C-His (HP866022) expressed in E. coli, spanning Ala2-Thr102. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9BW61
Protein lengthAla2-Thr102
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 39.89 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesC19orf58, DDA1, DET1- and DDB1-associated protein 1, PCIA-1, PCIA1, Placenta cross-immune reaction antigen 1
Background

DET1- and DDB1-associated protein 1 is a ~11 kDa protein. Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex.

1. Pick, E. et al. (2007) Molecular and cellular biology 27, 4708-19. PMID: 17452440
2. Han, T. et al. (2017) Science (New York, N.Y.) 356. PMID: 28302793
3. Uehara, T. et al. (2017) Nature chemical biology 13, 675-680. PMID: 28437394
4. Faust, TB. et al. (2020) Nature chemical biology 16, 7-14. PMID: 31686031
5. Bussiere, DE. et al. (2020) Nature chemical biology 16, 15-23. PMID: 31819272
Note For research use only
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Formula
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Formula
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