

| Catalog No. | HP866022 |
|---|---|
| Description |
Recombinant Human DDA1 Protein, N-GST & C-His (HP866022) expressed in E. coli, spanning Ala2-Thr102. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | Q9BW61 |
| Protein length | Ala2-Thr102 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 39.89 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | C19orf58, DDA1, DET1- and DDB1-associated protein 1, PCIA-1, PCIA1, Placenta cross-immune reaction antigen 1 |
| Background | DET1- and DDB1-associated protein 1 is a ~11 kDa protein. Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. In the DCX complexes, acts as a scaffolding subunit required to stabilize the complex. 1. Pick, E. et al. (2007) Molecular and cellular biology 27, 4708-19. PMID: 17452440 2. Han, T. et al. (2017) Science (New York, N.Y.) 356. PMID: 28302793 3. Uehara, T. et al. (2017) Nature chemical biology 13, 675-680. PMID: 28437394 4. Faust, TB. et al. (2020) Nature chemical biology 16, 7-14. PMID: 31686031 5. Bussiere, DE. et al. (2020) Nature chemical biology 16, 15-23. PMID: 31819272 |
| Note | For research use only |
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