Human DDAH2 Recombinant Protein (N-His) (HF506012)

Name: Human DDAH2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF506012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF506012
Description	Recombinant Human DDAH2 Protein, N-His (HF506012) expressed in E. coli, spanning Ala31-Leu265. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O95865
Protein length	Ala31-Leu265
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.08 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DDAH, Dimethylargininase-2, NG30, DDAHII, DDAH2, Protein G6a, N(G), N(G)-dimethylarginine dimethylaminohydrolase 2, S-phase protein, G6A, Dimethylarginine dimethylaminohydrolase 2, DDAH-2
Background	Putative hydrolase DDAH2 is a ~29 kDa protein. Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS. In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase. Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein. Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS. 1. Hu, X. et al. (2011) Arteriosclerosis, thrombosis, and vascular biology 31, 1540-6. PMID: 21493890 2. Ragavan, VN. et al. (2023) Nature communications 14, 3392. PMID: 37296100 3. Huang, S. et al. (2021) Science signaling 14. PMID: 33850055
Note	For research use only.

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SDS-PAGE for Recombinant Human DDAH2 Protein, N-His

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Datasheet

Human DDAH2 Recombinant Protein (N-His).pdf

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Human DDAH2 Recombinant Protein (N-His) (HF506012)

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