Human DDI2 Recombinant Protein (N-His) (HD875012)

Name: Human DDI2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD875012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD875012
Description	Recombinant Human DDI2 Protein, N-His (HD875012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q5TDH0
Protein length	Met1-Pro399
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DDI2, EC:3.4.23.-, Protein DDI1 homolog 2
Background	Protein DDI1 homolog 2 is a ~44 kDa protein. Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane. Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1. Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable). Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity. 1. Koizumi, S. et al. (2016) eLife 5. PMID: 27528193 2. Sha, Z. et al. (2016) Current biology : CB 26, R836-R837. PMID: 27676298 3. Kottemann, MC. et al. (2018) Molecular cell 69, 24-35.e5. PMID: 29290612
Note	For research use only

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Datasheet

Human DDI2 Recombinant Protein (N-His).pdf

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Human DDI2 Recombinant Protein (N-His) (HD875012)

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