Human DGAT1 Recombinant Protein (N-His) (HF844012)

Name: Human DGAT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF844012
Price: 211 USD
Availability: InStock

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Notice · Catalog HF844012 has been discontinued.

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Overview

Catalog No.	HF844012
Description	Recombinant Human DGAT1 Protein, N-His (HF844012) expressed in E. coli, spanning Ala180-Arg427. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O75907
Protein length	Ala180-Arg427
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.84 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ARAT, ACAT-related gene product 1, Diglyceride acyltransferase, Diacylglycerol O-acyltransferase 1, DGAT1, Retinol O-fatty-acyltransferase, Acyl-CoA retinol O-fatty-acyltransferase, AGRP1, DGAT
Background	Diacylglycerol O-acyltransferase 1 (DGAT1) is a ~55 kDa protein. Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. 1. Orland, MD. et al. (2005) Biochimica et biophysica acta 1737, 76-82. PMID: 16214399 2. Cheng, D. et al. (2008) The Journal of biological chemistry 283, 29802-11. PMID: 18768481 3. Ma, Z. et al. (2017) Journal of lipid research 58, 1091-1099. PMID: 28420705 4. Wang, L. et al. (2020) Nature 581, 329-332. PMID: 32433610 5. Sui, X. et al. (2020) Nature 581, 323-328. PMID: 32433611 6. Oelkers, P. et al. (1998) The Journal of biological chemistry 273, 26765-71. PMID: 9756920
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human DGAT1 Protein, N-His

References

Datasheet

Human DGAT1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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C₁ (Stock Conc.)

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Working Solution

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Human DGAT1 Recombinant Protein (N-His) (HF844012)

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