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Human DHRS7B Recombinant Protein (N-His) (HD321012)

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Overview
Catalog No.HD321012
Description
Recombinant Human DHRS7B Protein, N-His (HD321012) expressed in E. coli, spanning Arg39-Ser325. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ6IAN0
Protein lengthArg39-Ser325
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 33.36 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesDHRS7B, Dehydrogenase/reductase SDR family member 7B, EC:1.1.-.-, Protein SDR32C1, SDR32C1, Short-chain dehydrogenase/reductase family 32C member 1
Background

Peroxisomal reductase activating PPAR-gamma is a ~35 kDa protein. Reductase required for adipogenesis and activation of PPARG nuclear receptor. Can convert acyl and alkyl dihydroxyacetone-phosphate (DHAP) into glycerolipids and ether lipids, respectively, synthesizing ether-linked phospholipids that act as agonists for PPARG. Also regulates adipogenesis by preventing adipocyte browning: following import into the nucleus, interacts with PRDM16, inhibiting the interaction between PRDM16 and PPARG and preventing differentiation of brown adipocytes.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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