Human DHX16 Recombinant Protein (N-His) (HT408012)

Name: Human DHX16 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT408012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT408012
Description	Recombinant Human DHX16 Protein, N-His (HT408012) expressed in E. coli, spanning Glu364-Glu746. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60231
Protein length	Glu364-Glu746
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	44.96 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, 3.6.4.13, ATP-dependent RNA helicase #3, DEAH-box protein 16, DHX16, DBP2, DDX16, KIAA0577, PRP2
Background	Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 is a ~119 kDa protein. Required for pre-mRNA splicing as a component of the spliceosome. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Also plays a role in innate antiviral response by acting as a pattern recognition receptor sensing splicing signals in viral RNA. Mechanistically, TRIM6 promotes the interaction between unanchored 'Lys-48'-polyubiquitin chains and DHX16, leading to DHX16 interaction with RIGI and ssRNA to amplify RIGI-dependent innate antiviral immune responses. 1. Gencheva, M. et al. (2010) The Biochemical journal 429, 25-32. PMID: 20423332 2. Gencheva, M. et al. (2010) The Journal of biological chemistry 285, 35624-32. PMID: 20841358 3. Zang, S. et al. (2014) Bioscience reports 34, e00163. PMID: 25296192 4. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106 5. Hage, A. et al. (2022) Cell reports 38, 110434. PMID: 35263596
Note	For research use only.

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SDS-PAGE for Recombinant Human DHX16 Protein, N-His

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Datasheet

Human DHX16 Recombinant Protein (N-His).pdf

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Human DHX16 Recombinant Protein (N-His) (HT408012)

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