ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
125.95 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
KIAA1488, DHX36, MLE-like protein 1, RNA helicase associated with AU-rich element protein, G4R1, MLEL1, DEAD/H box polypeptide 36, ATP-dependent DNA/RNA helicase DHX36, RHAU, DDX36, DEAH-box protein 36, G4-resolvase-1
Background
ATP-dependent DNA/RNA helicase DHX36 (MLEL1) is a ~114 kDa protein. Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures. Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses. G4 structures correspond to helical structures containing guanine tetrads. Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-DNA and G4-RNA). Plays a role in genomic integrity.
1. Vaughn, JP. et al. (2005) The Journal of biological chemistry 280, 38117-20. PMID: 16150737
2. Chalupníková, K. et al. (2008) The Journal of biological chemistry 283, 35186-98. PMID: 18854321
3. Lattmann, S. et al. (2010) Nucleic acids research 38, 6219-33. PMID: 20472641
4. Giri, B. et al. (2011) Nucleic acids research 39, 7161-78. PMID: 21586581
5. Tran, H. et al. (2004) Molecular cell 13, 101-11. PMID: 14731398
6. Iwamoto, F. et al. (2008) Experimental cell research 314, 1378-91. PMID: 18279852
7. Huang, W. et al. (2012) Nucleic acids research 40, 1033-49. PMID: 21993297
8. Booy, EP. et al. (2012) Nucleic acids research 40, 4110-24. PMID: 22238380
9. Booy, EP. et al. (2015) Methods in molecular biology (Clifton, N.J.) 1259, 125-35. PMID: 25579584
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human DHX36/MLEL1 Protein, N-GST & C-His
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