Human DNAJC10 Recombinant Protein (N-His) (HD135012)

Name: Human DNAJC10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD135012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD135012
Description	Recombinant Human DNAJC10 Protein, N-His (HD135012) expressed in E. coli, spanning Ser354-Asn555. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8IXB1
Protein length	Ser354-Asn555
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.47 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNAJC10, DnaJ homolog subfamily C member 10, EC:1.8.4.-, ER-resident protein ERdj5, ERDJ5, ERdj5, Endoplasmic reticulum DNA J domain-containing protein 5, MTHr, Macrothioredoxin
Background	Endoplasmic reticulum disulfide reductase DNAJC10 is a ~91 kDa protein. Endoplasmic reticulum disulfide reductase that collaborates directly with the chaperone BIP/HSPA5 (GRP78) to maintain protein quality control by facilitating either the correct folding or the targeted degradation of misfolded proteins. Essential for efficient maturation of newly synthesized polypeptides in the endoplasmic reticulum, binds to substrate proteins and specifically catalyzes the reduction and removal of improper (non-native) disulfide bonds during the folding process. In endoplasmic reticulum-associated degradation (ERAD), DNAJC10 reduces incorrect disulfide bonds specifically in misfolded glycoproteins that have been recognized by EDEM1, a key component of the ERAD pathway, thereby enabling their retrotranslocation and degradation. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress. 1. Cunnea, PM. et al. (2003) The Journal of biological chemistry 278, 1059-66. PMID: 12411443 2. Oka, OB. et al. (2013) Molecular cell 50, 793-804. PMID: 23769672 3. Cai, X. et al. (2023) The Journal of biological chemistry 299, 105274. PMID: 37739037 4. Dong, M. et al. (2008) Molecular biology of the cell 19, 2620-30. PMID: 18400946 5. Thomas, CG. et al. (2009) The Journal of biological chemistry 284, 6282-90. PMID: 19122239
Note	For research use only

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Datasheet

Human DNAJC10 Recombinant Protein (N-His).pdf

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Human DNAJC10 Recombinant Protein (N-His) (HD135012)

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