Human DPPA3 Recombinant Protein (N-His-KSI) (HD361012)

Name: Human DPPA3 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HD361012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD361012
Description	Recombinant Human DPPA3 Protein, N-His-KSI (HD361012) expressed in E. coli, spanning Asp2-Pro159. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6W0C5
Protein length	Asp2-Pro159
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DPPA3, Developmental pluripotency-associated protein 3, STELLAR, Stella-related protein
Background	Developmental pluripotency-associated protein 3 is a ~17 kDa protein. Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. 1. Mazid, MA. et al. (2022) Nature 605, 315-324. PMID: 35314832
Note	For research use only

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Datasheet

Human DPPA3 Recombinant Protein (N-His-KSI).pdf

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Human DPPA3 Recombinant Protein (N-His-KSI) (HD361012)

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