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Human EHD3 Recombinant Protein (N-His) (HC245012)

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Overview
Catalog No.HC245012
Description
Recombinant Human EHD3 Protein, N-His (HC245012) expressed in E. coli, spanning Thr20-Ser402. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ9NZN3
Protein lengthThr20-Ser402
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 46.24 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEH domain-containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3
Background

EH domain-containing protein 3 is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid induces its membrane tubulation activity. Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport.

1. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250
2. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528
3. Naslavsky, N. et al. (2006) Molecular biology of the cell 17, 163-77. PMID: 16251358
4. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914
5. Naslavsky, N. et al. (2009) Journal of cell science 122, 389-400. PMID: 19139087
6. Waxmonsky, NC. et al. (2013) Journal of cell science 126, 3593-601. PMID: 23781025
7. Kotowski, SJ. et al. (2011) Neuron 71, 278-90. PMID: 21791287
8. Gudmundsson, H. et al. (2010) Circulation research 107, 84-95. PMID: 20489164
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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