Human EIF1 Recombinant Protein (C-His) (HD027012)

Name: Human EIF1 Recombinant Protein (C-His)
Brand: abinScience
SKU: HD027012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD027012
Description	Recombinant Human EIF1 Protein, C-His (HD027012) expressed in E. coli, spanning Met1-Phe113. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P41567
Protein length	Met1-Phe113
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	13.80 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	A121, EIF1, Eukaryotic translation initiation factor 1, Protein translation factor SUI1 homolog, SUI1, Sui1iso1, eIF1
Background	Eukaryotic translation initiation factor 1 is a ~12 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. Together with eIF1A (EIF1AX), EIF1 facilitates scanning and is essential for start codon recognition on the basis of AUG nucleotide context and location relative to the 5'-cap. Participates to initiation codon selection by influencing the conformation of the 40S ribosomal subunit and the positions of bound mRNA and initiator tRNA; this is possible after its binding to the interface surface of the platform of the 40S ribosomal subunit close to the P-site. Together with eIF1A (EIF1AX), also regulates the opening and closing of the mRNA binding channel, which ensures mRNA recruitment, scanning and the fidelity of initiation codon selection. Continuously monitors and protects against premature and partial base-pairing of codons in the 5'-UTR with the anticodon of initiator tRNA. 1. Pestova, TV. et al. (2002) Genes & development 16, 2906-22. PMID: 12435632 2. Lomakin, IB. et al. (2003) Genes & development 17, 2786-97. PMID: 14600024 3. Pestova, TV. et al. (1998) Nature 394, 854-9. PMID: 9732867 4. Haimov, O. et al. (2018) Molecular and cellular biology 38. PMID: 29987188 5. Luna, RE. et al. (2013) Biochemistry 52, 9510-8. PMID: 24319994 6. Luna, RE. et al. (2012) Cell reports 1, 689-702. PMID: 22813744
Note	For research use only

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Datasheet

Human EIF1 Recombinant Protein (C-His).pdf

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Human EIF1 Recombinant Protein (C-His) (HD027012)

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