Human EIF2AK4 Recombinant Protein (N-His) (HC773012)

Name: Human EIF2AK4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC773012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC773012
Description	Recombinant Human EIF2AK4 Protein, N-His (HC773012) expressed in E. coli, spanning Pro788-Thr1020. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9P2K8
Protein length	Pro788-Thr1020
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.78 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.7.11.1, EIF2AK4, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2, GCN2-like protein, KIAA1338, eIF-2-alpha kinase GCN2
Background	eIF-2-alpha kinase GCN2 is a ~186 kDa protein. Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha into a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs. Required for the translational induction of protein kinase PRKCH following amino acid starvation. 1. Roobol, A. et al. (2015) The Biochemical journal 465, 213-25. PMID: 25329545 2. Wu, CC. et al. (2020) Cell 182, 404-416.e14. PMID: 32610081 3. Lehman, SL. et al. (2015) PLoS genetics 11, e1005212. PMID: 26102367
Note	For research use only

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Datasheet

Human EIF2AK4 Recombinant Protein (N-His).pdf

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Human EIF2AK4 Recombinant Protein (N-His) (HC773012)

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