Human EIF5 Recombinant Protein (N-His) (HD092012)

Name: Human EIF5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD092012
Price: 211 USD
Availability: InStock

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Catalog No.	HD092012
Description	Recombinant Human EIF5 Protein, N-His (HD092012) expressed in E. coli, spanning Leu228-Glu386. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P55010
Protein length	Leu228-Glu386
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.06 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EIF5, Eukaryotic translation initiation factor 5, eIF-5
Background	Eukaryotic translation initiation factor 5 is a ~49 kDa protein. Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. 1. Paulin, FE. et al. (2001) Current biology : CB 11, 55-9. PMID: 11166181 2. Luna, RE. et al. (2012) Cell reports 1, 689-702. PMID: 22813744 3. Luna, RE. et al. (2013) Biochemistry 52, 9510-8. PMID: 24319994
Note	For research use only

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Human EIF5 Recombinant Protein (N-His).pdf

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Human EIF5 Recombinant Protein (N-His) (HD092012)

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