Human EIF5B Recombinant Protein (N-GST) (HC158012)

Name: Human EIF5B Recombinant Protein (N-GST)
Brand: abinScience
SKU: HC158012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC158012
Description	Recombinant Human EIF5B Protein, N-GST (HC158012) expressed in E. coli, spanning Thr871-Ile1220. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60841
Protein length	Thr871-Ile1220
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	66.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:3.6.5.3, EIF5B, Eukaryotic translation initiation factor 5B, IF2, KIAA0741, Translation initiation factor IF-2, eIF-5B
Background	Eukaryotic translation initiation factor 5B is a ~138 kDa protein. Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. 1. Pestova, TV. et al. (2000) Nature 403, 332-5. PMID: 10659855 2. Lapointe, CP. et al. (2022) Nature 607, 185-190. PMID: 35732735 3. Marintchev, A. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 1535-40. PMID: 12569173
Note	For research use only

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Datasheet

Human EIF5B Recombinant Protein (N-GST).pdf

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Human EIF5B Recombinant Protein (N-GST) (HC158012)

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