

| Catalog No. | HC250012 |
|---|---|
| Description |
Recombinant Human ELL/C19orf17 Protein, N-His-SUMO (HC250012) expressed in E. coli, spanning Gly199-Cys293. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | P55199 |
| Protein length | Gly199-Cys293 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 23.13 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | C19orf17, ELL, Eleven-nineteen lysine-rich leukemia protein, RNA polymerase II elongation factor ELL |
| Background | RNA polymerase II elongation factor ELL is a ~68 kDa protein. Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation. 1. Smith, ER. et al. (2011) Molecular cell 44, 954-65. PMID: 22195968 2. Hu, D. et al. (2013) Molecular cell 51, 493-505. PMID: 23932780 |
| Note | For research use only |
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