Human EMC6/TMEM93 Recombinant Protein (N-His-SUMO) (HP012012)

Name: Human EMC6/TMEM93 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HP012012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP012012
Description	Recombinant Human EMC6/TMEM93 Protein, N-His-SUMO (HP012012) expressed in E. coli, spanning Val4-Thr47. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BV81
Protein length	Val4-Thr47
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.88 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Transmembrane protein 93, TMEM93, EMC6, ER membrane protein complex subunit 6
Background	ER membrane protein complex subunit 6 (EMC6/TMEM93) is a ~12 kDa protein. Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. 1. Guna, A. et al. (2018) Science (New York, N.Y.) 359, 470-473. PMID: 29242231 2. Shurtleff, MJ. et al. (2018) eLife 7. PMID: 29809151 3. Chitwood, PJ. et al. (2018) Cell 175, 1507-1519.e16. PMID: 30415835 4. Pleiner, T. et al. (2020) Science (New York, N.Y.) 369, 433-436. PMID: 32439656 5. O'Donnell, JP. et al. (2020) eLife 9. PMID: 32459176
Note	For research use only.

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SDS-PAGE for Recombinant Human EMC6/TMEM93 Protein, N-His-SUMO

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Datasheet

Human EMC6/TMEM93 Recombinant Protein (N-His-SUMO).pdf

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Human EMC6/TMEM93 Recombinant Protein (N-His-SUMO) (HP012012)

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