Human ERCC6 Recombinant Protein (N-His) (HD168012)

Name: Human ERCC6 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD168012
Price: 211 USD
Availability: InStock

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Catalog No.	HD168012
Description	Recombinant Human ERCC6 Protein, N-His (HD168012) expressed in E. coli, spanning Gly456-Arg745. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q03468
Protein length	Gly456-Arg745
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATP-dependent helicase ERCC6, CSB, Cockayne syndrome protein CSB, DNA excision repair protein ERCC-6, EC:3.6.4.-, ERCC6
Background	DNA excision repair protein ERCC-6 is a ~168 kDa protein. Essential factor involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Plays a central role in the initiation of the TC-NER process: specifically recognizes and binds RNA polymerase II stalled at a lesion, and mediates recruitment of ERCC8/CSA, initiating DNA damage excision by TFIIH recruitment. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function. Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function. 1. Sarker, AH. et al. (2005) Molecular cell 20, 187-98. PMID: 16246722 2. Anindya, R. et al. (2010) Molecular cell 38, 637-48. PMID: 20541997 3. Bailey, AD. et al. (2012) DNA repair 11, 488-501. PMID: 22483866 4. Sin, Y. et al. (2016) The Journal of biological chemistry 291, 1387-97. PMID: 26620705 5. van der Weegen, Y. et al. (2020) Nature communications 11, 2104. PMID: 32355176 6. Kokic, G. et al. (2021) Nature 598, 368-372. PMID: 34526721 7. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879 8. Carnie, CJ. et al. (2024) Nature cell biology 26, 797-810. PMID: 38600235 9. van Sluis, M. et al. (2024) Nature cell biology 26, 770-783. PMID: 38600236
Note	For research use only

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Datasheet

Human ERCC6 Recombinant Protein (N-His).pdf

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Human ERCC6 Recombinant Protein (N-His) (HD168012)

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