Human F2R Recombinant Protein (N-GST) (HB844012)

Name: Human F2R Recombinant Protein (N-GST)
Brand: abinScience
SKU: HB844012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB844012
Description	Recombinant Human F2R Protein, N-GST (HB844012) expressed in E. coli, spanning Ser42-Thr102. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P25116
Protein length	Ser42-Thr102
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.89 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Proteinase-activated receptor 1, PAR-1, PAR1, CF2R, Coagulation factor II receptor, Thrombin receptor, F2R, TR
Background	Proteinase-activated receptor 1 (F2R/PAR1) is a ~47 kDa protein. High affinity receptor that binds the activated thrombin, leading to calcium release from intracellular stores. The thrombin-activated receptor signaling pathway is mediated through PTX-insensitive G proteins, activation of phospholipase C resulting in the production of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) which binds to InsP3 receptors causing calcium release from the stores. In astrocytes, the calcium released into the cytosol allows the Ca(2+)-dependent release of L-glutamate into the synaptic cleft through BEST1, that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation. May play a role in platelets activation and in vascular development. Mediates up-regulation of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL6, triggered by coagulation factor Xa (F10) in cardiac fibroblasts and umbilical vein endothelial cells. 1. Vu, TK. et al. (1991) Cell 64, 1057-68. PMID: 1672265 2. Mathews, II. et al. (1994) Biochemistry 33, 3266-79. PMID: 8136362 3. Kahn, ML. et al. (1999) The Journal of clinical investigation 103, 879-87. PMID: 10079109 4. Shinozawa, E. et al. (2018) Frontiers in pharmacology 9, 1431. PMID: 30568593 5. D'Alessandro, E. et al. (2021) Cells 10. PMID: 34831181
Note	For research use only.

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SDS-PAGE for Recombinant Human F2R Protein, N-GST

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Datasheet

Human F2R Recombinant Protein (N-GST).pdf

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Human F2R Recombinant Protein (N-GST) (HB844012)

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