Human FFAR2 Recombinant Protein (N-GST & C-His) (HC898012)

Name: Human FFAR2 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC898012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC898012
Description	Recombinant Human FFAR2 Protein, N-GST & C-His (HC898012) expressed in E. coli, spanning Ser277-Glu330. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15552
Protein length	Ser277-Glu330
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	FFA2, FFAR2, Free fatty acid receptor 2, G-protein coupled receptor 43, GPCR43, GPR43
Background	Free fatty acid receptor 2 is a ~37 kDa protein. G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family. Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. 1. Brown, AJ. et al. (2003) The Journal of biological chemistry 278, 11312-9. PMID: 12496283 2. Le Poul, E. et al. (2003) The Journal of biological chemistry 278, 25481-9. PMID: 12711604 3. Hudson, BD. et al. (2013) The Journal of biological chemistry 288, 17296-312. PMID: 23589301 4. Zhang, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 6899-910. PMID: 21037097 5. Hudson, BD. et al. (2012) The Journal of biological chemistry 287, 41195-209. PMID: 23066016
Note	For research use only

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Datasheet

Human FFAR2 Recombinant Protein (N-GST & C-His).pdf

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Human FFAR2 Recombinant Protein (N-GST & C-His) (HC898012)

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