Human Flt-3 Ligand/FLT3L Recombinant Protein (C-His) (HW688011)

Name: Human Flt-3 Ligand/FLT3L Recombinant Protein (C-His)
Brand: abinScience
SKU: HW688011
Price: 264 USD
Availability: InStock

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Overview

Catalog No.	HW688011
Description	Recombinant Human Flt3 ligand/FLT3LG Protein, C-His (HW688011) expressed in Insect Cells, spanning Ser25-Pro184. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Insect cells
Accession	P49771
Protein length	Ser25-Pro184
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.05 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SL cytokine, FLT3LG, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L
Background	Fms-related tyrosine kinase 3 ligand (Flt3 ligand/FLT3LG) is a ~26 kDa protein. Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Required for the development of B cells, and dendritic cells (DCs).
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human Flt3 ligand/FLT3LG Protein, C-His
Bioactivity

Detected by Iv0057 (Catalog No: HW688010) in indirect ELISA

References

Datasheet

Human Flt-3 Ligand/FLT3L Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

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Working Solution

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Human Flt-3 Ligand/FLT3L Recombinant Protein (C-His) (HW688011)

Overview

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References

Datasheet

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