Human FOXK1 Recombinant Protein (N-His) (HB309012)

Name: Human FOXK1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB309012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB309012
Description	Recombinant Human FOXK1 Protein, N-His (HB309012) expressed in E. coli, spanning Ala86-Arg395. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P85037
Protein length	Ala86-Arg395
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.31 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Forkhead box protein K1; Myocyte nuclear factor; MNF; FOXK1; MNF
Background	Forkhead box protein K1 is a ~75 kDa protein. Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy. Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context. Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen. Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4. Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate. 1. Freddie, CT. et al. (2007) Nucleic acids research 35, 5203-12. PMID: 17670796 2. Wang, W. et al. (2015) Developmental cell 32, 707-18. PMID: 25805136 3. Panda, D. et al. (2015) mBio 6. PMID: 25852164 4. Baymaz, HI. et al. (2014) Proteomics 14, 2179-89. PMID: 24634419 5. Campagne, A. et al. (2019) Nature communications 10, 348. PMID: 30664650 6. Zhang, HL. et al. (2025) Nature chemical biology 21, 1531-1543. PMID: 40246981
Note	For research use only

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SDS-PAGE for Recombinant Human FOXK1 Protein, N-His

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Datasheet

Human FOXK1 Recombinant Protein (N-His).pdf

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Human FOXK1 Recombinant Protein (N-His) (HB309012)

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