Recombinant Human FTO Protein, C-His (HP668011) expressed in Mammalian Cells, spanning Met1-Pro505. Purity: >90% by SDS-PAGE.
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Highlights
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●His-Tagged — N-terminal 6×His tag for IMAC purification.
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●Mammalian Expression — Proper folding and native glycosylation.
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●High Purity — >90% purity verified by SDS-PAGE.
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Expression system
Mammalian cells
Accession
Q9C0B1
Protein length
Met1-Pro505
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
59.25 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alpha-ketoglutarate-dependent dioxygenase FTO is a ~58 kDa protein. RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA.
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1. Jia, G. et al. (2011) Nature chemical biology 7, 885-7. PMID: 22002720
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2. Huang, Y. et al. (2015) Nucleic acids research 43, 373-84. PMID: 25452335
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3. Wang, T. et al. (2015) Journal of the American Chemical Society 137, 13736-9. PMID: 26457839
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4. Zhou, J. et al. (2015) Nature 526, 591-4. PMID: 26458103
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5. Mauer, J. et al. (2017) Nature 541, 371-375. PMID: 28002401
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6. Wei, J. et al. (2018) Molecular cell 71, 973-985.e5. PMID: 30197295
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