Human Galectin-8/GAL8 Recombinant Protein (N-His & C-Flag) (HT448011)

Name: Human Galectin-8/GAL8 Recombinant Protein (N-His & C-Flag)
Brand: abinScience
SKU: HT448011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HT448011
Description	Recombinant Human Galectin-8/GAL8 Protein, N-His & C-Flag (HT448011) expressed in Mammalian Cells, spanning Met1-Trp317. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	O00214
Protein length	Met1-Trp317
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.90 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PCTA-1, Po66 carbohydrate-binding protein, Po66-CBP, Galectin-8, Gal-8, LGALS8, Prostate carcinoma tumor antigen 1
Background	Galectin-8 (GAL-8) is a ~35 kDa protein. Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. 1. Thurston, TL. et al. (2012) Nature 482, 414-8. PMID: 22246324 2. Staring, J. et al. (2017) Nature 541, 412-416. PMID: 28077878 3. Ideo, H. et al. (2011) The Journal of biological chemistry 286, 11346-55. PMID: 21288902
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human Galectin-8/GAL8 Protein, N-His & C-Flag

References

Datasheet

Human Galectin-8/GAL8 Recombinant Protein (N-His & C-Flag).pdf

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Formula

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Human Galectin-8/GAL8 Recombinant Protein (N-His & C-Flag) (HT448011)

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References

Datasheet

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