Human GATA4 Recombinant Protein (N-His) (HW583012)

Name: Human GATA4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW583012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW583012
Description	Recombinant Human GATA4 Protein, N-His (HW583012) expressed in E. coli, spanning Arg201-Ala442. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P43694
Protein length	Arg201-Ala442
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GATA4, GATA-binding factor 4, Transcription factor GATA-4
Background	Transcription factor GATA-4 (GATA4) is a ~44 kDa protein. Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. 1. Yang, YQ. et al. (2013) Human mutation 34, 1662-71. PMID: 24000169 2. Ang, YS. et al. (2016) Cell 167, 1734-1749.e22. PMID: 27984724 3. Gonzalez-Teran, B. et al. (2022) Cell 185, 794-814.e30. PMID: 35182466 4. Sunagawa, Y. et al. (2010) The Journal of biological chemistry 285, 9556-9568. PMID: 20081228 5. Lourenço, D. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 1597-602. PMID: 21220346 6. Oskouian, B. et al. (2005) The Journal of biological chemistry 280, 18403-10. PMID: 15734735
Note	For research use only.

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SDS-PAGE for Recombinant Human GATA4 Protein, N-His

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Datasheet

Human GATA4 Recombinant Protein (N-His).pdf

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Human GATA4 Recombinant Protein (N-His) (HW583012)

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