Human GCKR Recombinant Protein (N-His) (HD217012)

Name: Human GCKR Recombinant Protein (N-His)
Brand: abinScience
SKU: HD217012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD217012
Description	Recombinant Human GCKR Protein, N-His (HD217012) expressed in E. coli, spanning Met1-Asn503. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14397
Protein length	Met1-Asn503
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	57.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GCKR, GKRP, Glucokinase regulator, Glucokinase regulatory protein
Background	Glucokinase regulatory protein is a ~68 kDa protein. Regulates glucokinase (GCK) by forming an inactive complex with this enzyme. Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity. 1. Pautsch, A. et al. (2013) Biochemistry 52, 3523-31. PMID: 23621087 2. Choi, JM. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10171-6. PMID: 23733961 3. de la Iglesia, N. et al. (1999) FEBS letters 456, 332-8. PMID: 10456334
Note	For research use only

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Datasheet

Human GCKR Recombinant Protein (N-His).pdf

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Human GCKR Recombinant Protein (N-His) (HD217012)

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