Human GFI1 Recombinant Protein (N-His) (HD909012)

Name: Human GFI1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD909012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD909012
Description	Recombinant Human GFI1 Protein, N-His (HD909012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q99684
Protein length	Lys256-Lys422
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GFI1, Growth factor independent protein 1, ZNF163, Zinc finger protein 163, Zinc finger protein Gfi-1
Background	Zinc finger protein Gfi-1 is a ~45 kDa protein. Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. 1. Rödel, B. et al. (2000) The EMBO journal 19, 5845-55. PMID: 11060035 2. Dahl, R. et al. (2007) The Journal of biological chemistry 282, 6473-83. PMID: 17197705 3. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546 4. Montoya-Durango, DE. et al. (2008) The Journal of biological chemistry 283, 32056-65. PMID: 18805794 5. Basu, S. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 1433-8. PMID: 19164764 6. Liu, Q. et al. (2010) Oncogene 29, 2843-52. PMID: 20190815 7. Zweidler-Mckay, PA. et al. (1996) Molecular and cellular biology 16, 4024-34. PMID: 8754800 10. Sharif-Askari, E. et al. (2010) Molecular and cellular biology 30, 3929-42. PMID: 20547752
Note	For research use only

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Datasheet

Human GFI1 Recombinant Protein (N-His).pdf

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Human GFI1 Recombinant Protein (N-His) (HD909012)

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