Human GPR183 Recombinant Protein (N-His-SUMO) (HB793012)

Name: Human GPR183 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HB793012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB793012
Description	Recombinant Human GPR183 Protein, N-His-SUMO (HB793012) expressed in E. coli, spanning Lys313-Lys361. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P32249
Protein length	Lys313-Lys361
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GPR183, G-protein coupled receptor 183, hEBI2, Epstein-Barr virus-induced G-protein coupled receptor 2, EBI2, EBV-induced G-protein coupled receptor 2
Background	G-protein coupled receptor 183 (GPR183) is a ~41 kDa protein. G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions. 1. Hannedouche, S. et al. (2011) Nature 475, 524-7. PMID: 21796212 2. Benned-Jensen, T. et al. (2012) The Journal of biological chemistry 287, 35470-35483. PMID: 22875855 3. Zhang, L. et al. (2012) Molecular pharmacology 82, 1094-103. PMID: 22930711 4. Barroso, R. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 4841-54. PMID: 22913878 5. Rutkowska, A. et al. (2015) Glia 63, 341-51. PMID: 25297897 6. Benned-Jensen, T. et al. (2011) The Journal of biological chemistry 286, 29292-29302. PMID: 21673108
Note	For research use only.