Human GPR4 Recombinant Protein (N-His-KSI) (HA696012)

Name: Human GPR4 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HA696012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA696012
Description	Recombinant Human GPR4 Protein, N-His-KSI (HA696012) expressed in E. coli, spanning Ile284-Gln362. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P46093
Protein length	Ile284-Gln362
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.26 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	G-protein coupled receptor 4; G-protein coupled receptor 6C.l; GPR6C.l; GPR4
Background	G-protein coupled receptor 4 is a ~40 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. Activated by an optimal pH of 6.8-7.2. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. GPR4 is mainly coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. May also couple with G(i), G(q) and G(12)/G(13) G proteins. 1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148 2. Tobo, M. et al. (2007) Cellular signalling 19, 1745-53. PMID: 17462861 3. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938 4. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132 5. Ma, Y. et al. (2025) Nature communications 16, 605. PMID: 39799123 6. Yue, X. et al. (2025) Cell research 35, 423-436. PMID: 40211064 7. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959 8. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960
Note	For research use only

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SDS-PAGE for Recombinant Human GPR4 Protein, N-His-KSI

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Datasheet

Human GPR4 Recombinant Protein (N-His-KSI).pdf

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Human GPR4 Recombinant Protein (N-His-KSI) (HA696012)

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