Human GPR68 Recombinant Protein (N-His-KSI) (HS932012)

Name: Human GPR68 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HS932012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HS932012
Description	Recombinant Human GPR68 Protein, N-His-KSI (HS932012) expressed in E. coli, spanning Leu285-Ala365. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15743
Protein length	Leu285-Ala365
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.31 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ovarian cancer G-protein coupled receptor 1; OGR-1; G-protein coupled receptor 68; GPR12A; Sphingosylphosphorylcholine receptor; GPR68; OGR1
Background	G-protein coupled receptor 68 is a ~41 kDa protein. Proton-sensing G-protein coupled receptor activated by extracellular pH, which is required to monitor pH changes and generate adaptive reactions. The receptor is almost silent at pH 7.8 but fully activated at pH 6.8. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as phospholipase C. GPR68 is mainly coupled to G(q) G proteins and mediates production of diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Acts as a key mechanosensor of fluid shear stress and membrane stretch. 1. Ludwig, MG. et al. (2003) Nature 425, 93-8. PMID: 12955148 2. Xu, J. et al. (2018) Cell 173, 762-775.e16. PMID: 29677517 3. Huang, XP. et al. (2020) Biochemistry 59, 3594-3614. PMID: 32865988 4. Rowe, JB. et al. (2021) The Journal of biological chemistry 296, 100167. PMID: 33478938 5. Howard, MK. et al. (2025) Cell 188, 671-687.e20. PMID: 39753132 6. Guo, L. et al. (2025) Molecular cell 85, 1658-1673.e7. PMID: 40215959 7. Chen, LN. et al. (2025) Molecular cell 85, 1640-1657.e8. PMID: 40215960
Note	For research use only

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SDS-PAGE for Recombinant Human GPR68 Protein, N-His-KSI

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Datasheet

Human GPR68 Recombinant Protein (N-His-KSI).pdf

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Human GPR68 Recombinant Protein (N-His-KSI) (HS932012)

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