Human GPS2 Recombinant Protein (N-His-KSI) (HB469012)

Name: Human GPS2 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: HB469012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB469012
Description	Recombinant Human GPS2 Protein, N-His-KSI (HB469012) expressed in E. coli, spanning Pro2-Lys327. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13227
Protein length	Pro2-Lys327
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	52.32 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	G protein pathway suppressor 2; GPS-2; GPS2
Background	G protein pathway suppressor 2 is a ~36 kDa protein. Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'-linked ubiquitination. In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context. Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation. Promotes cholesterol efflux by acting as a transcription coactivator. Acts as a regulator of B-cell development by inhibiting UBE2N/Ubc13, thereby restricting the activation of Toll-like receptors (TLRs) and B-cell antigen receptors (BCRs) signaling pathways. 1. Bi, H. et al. (2014) Molecular biology of the cell 25, 2499-508. PMID: 24943844 2. Jakobsson, T. et al. (2009) Molecular cell 34, 510-8. PMID: 19481530 3. Cardamone, MD. et al. (2018) Molecular cell 69, 757-772.e7. PMID: 29499132 4. Cheng, X. et al. (2009) The Journal of biological chemistry 284, 36395-36404. PMID: 19858209 5. Venteclef, N. et al. (2010) Genes & development 24, 381-95. PMID: 20159957 6. Spain, BH. et al. (1996) Molecular and cellular biology 16, 6698-706. PMID: 8943324 7. Huang, XD. et al. (2016) Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 37, 13333-13343. PMID: 27460081
Note	For research use only

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SDS-PAGE for Recombinant Human GPS2 Protein, N-His-KSI

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Datasheet

Human GPS2 Recombinant Protein (N-His-KSI).pdf

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Human GPS2 Recombinant Protein (N-His-KSI) (HB469012)

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