Human GRK2 Recombinant Protein (N-GST) (HB035012)

Name: Human GRK2 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HB035012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB035012
Description	Recombinant Human GRK2 Protein, N-GST (HB035012) expressed in E. coli, spanning Asp398-Leu640. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P25098
Protein length	Asp398-Leu640
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	55.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	G-protein coupled receptor kinase 2, GRK2, ADRBK1, Beta-adrenergic receptor kinase 1, BARK1, BARK, Beta-ARK-1
Background	Beta-adrenergic receptor kinase 1 (GRK2) is a ~79 kDa protein. Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Phosphorylates catecholamine-activated ADRB2 to regulate physiological cardiomyocyte contraction rate responses. Also phosphorylates ligand-bound C3a and C5a anaphylatoxin receptors (C3AR1 and C5AR1, respectively), leading to receptor desensitization. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. 1. Pao, CS. et al. (2009) Biochemistry 48, 7325-33. PMID: 19715378 2. Guo, Q. et al. (2011) PloS one 6, e22559. PMID: 21799898 3. Gupta, K. et al. (2012) PloS one 7, e46369. PMID: 23077507 4. Aziziyeh, AI. et al. (2009) Cellular signalling 21, 1207-17. PMID: 19306925 5. Yim, PD. et al. (2019) American journal of physiology. Lung cellular and molecular physiology 316, L82-L93. PMID: 30284927
Note	For research use only.

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SDS-PAGE for Recombinant Human GRK2 Protein, N-GST

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Datasheet

Human GRK2 Recombinant Protein (N-GST).pdf

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Human GRK2 Recombinant Protein (N-GST) (HB035012)

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