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Human GTF2B Recombinant Protein (N-His) (HD836012)

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Overview
Catalog No.HD836012
Description
Recombinant Human GTF2B Protein, N-His (HD836012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ00403
Protein lengthSer108-Leu316
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:2.3.1.48, GTF2B, General transcription factor TFIIB, S300-II, TF2B, TFIIB, Transcription initiation factor IIB
Background

Transcription initiation factor IIB is a ~34 kDa protein. General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions.

1. Choi, CH. et al. (2003) Nature 424, 965-9. PMID: 12931194
2. Ing, NH. et al. (1992) The Journal of biological chemistry 267, 17617-23. PMID: 1517211
3. Ha, I. et al. (1991) Nature 352, 689-95. PMID: 1876184
4. Malik, S. et al. (1991) Proceedings of the National Academy of Sciences of the United States of America 88, 9553-7. PMID: 1946368
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3310-21. PMID: 3029109
7. Reinberg, D. et al. (1987) The Journal of biological chemistry 262, 3322-30. PMID: 3818643
8. Zawel, L. et al. (1995) Genes & development 9, 1479-90. PMID: 7601352
9. Malik, S. et al. (1993) Molecular and cellular biology 13, 6253-9. PMID: 8413225
10. Hisatake, K. et al. (1993) Nature 363, 744-7. PMID: 8515820
Note For research use only
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Formula
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