Human HIC1 Recombinant Protein (N-His) (HA798012)

Name: Human HIC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA798012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA798012
Description	Recombinant Human HIC1 Protein, N-His (HA798012) expressed in E. coli, spanning Lys561-Thr733. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14526
Protein length	Lys561-Thr733
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.55 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Hypermethylated in cancer 1 protein; Hic-1; Zinc finger and BTB domain-containing protein 29; HIC1; ZBTB29
Background	Hypermethylated in cancer 1 protein is a ~76 kDa protein. Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. Involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. 1. Deltour, S. et al. (2002) Molecular and cellular biology 22, 4890-901. PMID: 12052894 2. Pinte, S. et al. (2004) The Journal of biological chemistry 279, 38313-24. PMID: 15231840 3. Zhang, W. et al. (2010) Oncogene 29, 2467-76. PMID: 20154726 4. Chen, WY. et al. (2005) Cell 123, 437-48. PMID: 16269335 5. Van Rechem, C. et al. (2010) Molecular and cellular biology 30, 4045-59. PMID: 20547755 6. Valenta, T. et al. (2006) The EMBO journal 25, 2326-37. PMID: 16724116 7. Briggs, KJ. et al. (2008) Genes & development 22, 770-85. PMID: 18347096
Note	For research use only

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SDS-PAGE for Recombinant Human HIC1 Protein, N-His

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Datasheet

Human HIC1 Recombinant Protein (N-His).pdf

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Human HIC1 Recombinant Protein (N-His) (HA798012)

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