Human HSPG2 Recombinant Protein (N-His) (HX030012)

Name: Human HSPG2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX030012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX030012
Description	Recombinant Human HSPG2 Protein, N-His (HX030012) expressed in E. coli, spanning Leu4149-Ser4391. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P98160
Protein length	Leu4149-Ser4391
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	HSPG2, Perlecan, Basement membrane-specific heparan sulfate proteoglycan core protein, HSPG, PLC
Background	Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2) is a ~468 kDa protein. Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. 1. Mongiat, M. et al. (2003) The Journal of biological chemistry 278, 4238-49. PMID: 12435733 2. Gonzalez, EM. et al. (2005) The Journal of biological chemistry 280, 7080-7. PMID: 15591058 3. Nyström, A. et al. (2009) Blood 114, 4897-906. PMID: 19789387
Note	For research use only.

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SDS-PAGE for Recombinant Human HSPG2 Protein, N-His

References

Datasheet

Human HSPG2 Recombinant Protein (N-His).pdf

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Formula

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Human HSPG2 Recombinant Protein (N-His) (HX030012)

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