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Human IBSP Recombinant Protein (N-His-KSI) (HD788012)

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Overview
Catalog No.HD788012
Description
Recombinant Human IBSP Protein, N-His-KSI (HD788012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP21815
Protein lengthPhe17-Gln317
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesBNSP, BSP II, Bone sialoprotein 2, Bone sialoprotein II, Cell-binding sialoprotein, IBSP, Integrin-binding sialoprotein
Background

Integrin-binding sialoprotein is a ~35 kDa protein. Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes adhesion and migration of various cells via the alpha-V/beta-3 integrin receptor (ITGAV:ITGB3).

1. Wuttke, M. et al. (2001) The Journal of biological chemistry 276, 36839-48. PMID: 11459848
2. Bianco, P. et al. (1991) Calcified tissue international 49, 421-6. PMID: 1818768
3. Byzova, TV. et al. (2000) Experimental cell research 254, 299-308. PMID: 10640428
4. Rapuano, BE. et al. (2013) European journal of oral sciences 121, 600-9. PMID: 24103036
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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