Human IGFBP5 Recombinant Protein (N-His-SUMO&C-Strep) (HC001012)

Name: Human IGFBP5 Recombinant Protein (N-His-SUMO&C-Strep)
Brand: abinScience
SKU: HC001012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC001012
Description	Recombinant Human IGFBP5 Protein, N-His-SUMO&C-Strep (HC001012) expressed in E. coli, spanning Cys30-Glu121. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P24593
Protein length	Cys30-Glu121
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.83 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; IGFBP5; IBP5
Background	Insulin-like growth factor-binding protein 5 is a ~30 kDa protein. Multifunctional protein that plays a critical role in regulating the availability of IGFs to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Increases the cell proliferation of osteoblasts, intestinal smooth muscle cells and neuroblastoma cells. Enhances adhesion and survival of epithelial cells but decreases adhesion of mesenchymal cells. Once secreted, acts as a major mediator of mTORC1-dependent feedback inhibition of IGF1 signaling. Also plays a role in the induction of extracellular matrix (ECM) production and deposition independently of its nuclear translocation and binding to IGFs. 1. Busby, WH. et al. (2009) Osteoarthritis and cartilage 17, 547-55. PMID: 18930415 2. Jones, JI. et al. (1993) The Journal of cell biology 121, 679-87. PMID: 7683690 3. Yamaguchi, Y. et al. (2011) Journal of cellular and molecular medicine 15, 957-69. PMID: 20345844 4. Su, Y. et al. (2015) PloS one 10, e0130546. PMID: 26103640 5. Lin, W. et al. (2023) Nature communications 14, 1578. PMID: 36949068
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human IGFBP5 Protein, N-His-SUMO&C-Strep

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Datasheet

Human IGFBP5 Recombinant Protein (N-His-SUMO&C-Strep).pdf

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Human IGFBP5 Recombinant Protein (N-His-SUMO&C-Strep) (HC001012)

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