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Human IgG Recombinant Protein (HV080021)

Human IgG Recombinant Protein
Human IgG Recombinant Protein
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  • Overview

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  • References

  • Datasheet

  • SDS

Overview
Catalog No.HV080021
Description
Recombinant Human IgG Protein (HV080021) expressed in Mammalian Cells. Purity: >90% by SDS-PAGE.
Highlights
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Protein lengthFull length.
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Host speciesHuman
ClonalityMonoclonal
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesIg gamma-1 chain C region EU, Immunoglobulin heavy constant gamma 1, IGHG1, Ig gamma-1 chain C region NIE, Ig gamma-1 chain C region, Ig gamma-1 chain C region KOL
Background

Immunoglobulin heavy constant gamma 1 (IgG) is a ~43 kDa protein. Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain.

1. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
2. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
3. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
Note For research use only.
Images
  • Human IgG Recombinant Protein

    SDS-PAGE

    SDS-PAGE for Recombinant Human IgG Protein

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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