ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
53.06 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
CPSF3-like protein, CPSF3L, Cleavage and polyadenylation-specific factor 3-like protein, EC:3.1.27.-, INTS11, Int11, Integrator complex subunit 11, Protein related to CPSF subunits of 68 kDa, RC-68, RC68
Background
Integrator complex subunit 11 is a ~67 kDa protein. RNA endonuclease component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Within the integrator complex, INTS11 constitutes the RNA endonuclease subunit that degrades exiting nascent RNA transcripts. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex.
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2. Gardini, A. et al. (2014) Molecular cell 56, 128-139. PMID: 25201415
3. Wu, Y. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 4394-4399. PMID: 28396433
4. Beckedorff, F. et al. (2020) Cell reports 32, 107917. PMID: 32697989
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8. Hu, S. et al. (2023) Molecular cell 83, 1588-1604.e5. PMID: 37080207
9. Fianu, I. et al. (2024) Nature 629, 219-227. PMID: 38570683
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