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Human IPMK Recombinant Protein (N-His) (HC789012)

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Overview
Catalog No.HC789012
Description
Recombinant Human IPMK Protein, N-His (HC789012) expressed in E. coli, spanning Gly64-Ser260. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ8NFU5
Protein lengthGly64-Ser260
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 25.30 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:2.7.1.140, EC:2.7.1.151, EC:2.7.1.153, IMPK, IPMK, Inositol 1,3,4,6-tetrakisphosphate 5-kinase, Inositol polyphosphate multikinase
Background

Inositol polyphosphate multikinase is a ~47 kDa protein. Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates inositol 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate.

1. Nalaskowski, MM. et al. (2002) The Biochemical journal 366, 549-56. PMID: 12027805
2. Chang, SC. et al. (2002) The Journal of biological chemistry 277, 43836-43. PMID: 12223481
3. Wang, H. et al. (2017) The Journal of biological chemistry 292, 18192-18202. PMID: 28882892
4. Seacrist, CD. et al. (2018) Scientific reports 8, 16672. PMID: 30420721
5. Gu, C. et al. (2019) Journal of medicinal chemistry 62, 1443-1454. PMID: 30624931
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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