ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
34.56 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
MYST-1, hMOF, Histone acetyltransferase KAT8, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, KAT8, MYST1, MOF, Lysine acetyltransferase 8
Background
Histone acetyltransferase KAT8 is a ~52 kDa protein. Histone acetyltransferase that catalyzes histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) or 'Lys-16' (H4K16ac), depending on the context. Catalytic component of the MSL histone acetyltransferase complex, a multiprotein complex that mediates the majority of histone H4 acetylation at 'Lys-16' (H4K16ac), an epigenetic mark that prevents chromatin compaction. H4K16ac constitutes the only acetylation mark intergenerationally transmitted and regulates key biological processes, such as oogenesis, embryonic stem cell pluripotency, hematopoiesis or glucose metabolism. The MSL complex is required for chromosome stability and genome integrity by maintaining homeostatic levels of H4K16ac. The MSL complex is also involved in gene dosage by promoting up-regulation of genes expressed by the X chromosome.
1. Pardo, PS. et al. (2002) The Journal of biological chemistry 277, 50860-6. PMID: 12397079
2. Smith, ER. et al. (2005) Molecular and cellular biology 25, 9175-88. PMID: 16227571
3. Mendjan, S. et al. (2006) Molecular cell 21, 811-23. PMID: 16543150
4. Cai, Y. et al. (2010) The Journal of biological chemistry 285, 4268-72. PMID: 20018852
5. Kadlec, J. et al. (2011) Nature structural & molecular biology 18, 142-9. PMID: 21217699
6. Yuan, H. et al. (2012) The EMBO journal 31, 58-70. PMID: 22020126
7. Huang, J. et al. (2012) Cell research 22, 1078-81. PMID: 22547026
8. Li, L. et al. (2020) The Journal of clinical investigation 130, 1431-1445. PMID: 31794431
9. Monserrat, J. et al. (2021) Nature cell biology 23, 401-412. PMID: 33837287
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human KAT8 Protein, N-His
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