Human KCNJ10 Recombinant Protein (N-His) (HX144012)

Name: Human KCNJ10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX144012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX144012
Description	Recombinant Human KCNJ10 Protein, N-His (HX144012) expressed in E. coli, spanning Phe165-Val379. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P78508
Protein length	Phe165-Val379
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.08 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATP-dependent inwardly rectifying potassium channel Kir4.1, KCNJ10, ATP-sensitive inward rectifier potassium channel 10, Inward rectifier K(+) channel Kir1.2, Potassium channel, inwardly rectifying subfamily J member 10
Background	ATP-sensitive inward rectifier potassium channel 10 (KCNJ10) is a ~42 kDa protein. May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. 1. Shuck, ME. et al. (1997) The Journal of biological chemistry 272, 586-93. PMID: 8995301 2. Tanemoto, M. et al. (2014) FEBS letters 588, 899-905. PMID: 24561201
Note	For research use only.

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SDS-PAGE for Recombinant Human KCNJ10 Protein, N-His

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Datasheet

Human KCNJ10 Recombinant Protein (N-His).pdf

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Formula

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Human KCNJ10 Recombinant Protein (N-His) (HX144012)

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