

| Catalog No. | HY379032 |
|---|---|
| Description |
Recombinant Human LAMC1 Protein, N-His (HY379032) expressed in E. coli, spanning Gly70-Lys287. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | P11047 |
| Protein length | Gly70-Lys287 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 26.67 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Laminin-7 subunit gamma, Laminin-1 subunit gamma, Laminin subunit gamma-1, Laminin-10 subunit gamma, Laminin-2 subunit gamma, S-laminin subunit gamma, LAMC1, Laminin-11 subunit gamma, Laminin-3 subunit gamma, S-LAM gamma, Laminin-6 subunit gamma, Laminin-9 subunit gamma, Laminin-4 subunit gamma, Laminin-8 subunit gamma, LAMB2, Laminin B2 chain, laminin 1, laminin 111, EHS laminin, laminin 2, laminin 211, merosin, laminin 3, laminin 121, S laminin, laminin 4, laminin 221, S merosin, laminin 6, laminin 311, K laminin, laminin 7, laminin 321, KS laminin, laminin 8, laminin 411, laminin 9, laminin 421, laminin 10, laminin 511, laminin 11, laminin 521 |
| Background | Laminin subunit gamma-1 (LAMC1) is a ~177 kDa protein. Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway. |
| Note | For research use only. |

SDS-PAGE for Recombinant Human LAMC1 Protein, N-His




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