Human LBR Recombinant Protein (N-GST & C-His) (HC667012)

Name: Human LBR Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC667012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC667012
Description	Recombinant Human LBR Protein, N-GST & C-His (HC667012) expressed in E. coli, spanning Ala7-Arg61. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14739
Protein length	Ala7-Arg61
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.50 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	LMN2R, LBR, C14SR, Integral nuclear envelope inner membrane protein, Sterol C14-reductase, 3-beta-hydroxysterol Delta (14)-reductase, Delta(14)-sterol reductase LBR, Lamin-B receptor, Delta-14-SR, C-14 sterol reductase
Background	Delta(14)-sterol reductase LBR is a ~70 kDa protein. Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis. Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation. Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation. Anchors the lamina and the heterochromatin to the inner nuclear membrane. 1. Waterham, HR. et al. (2003) American journal of human genetics 72, 1013-7. PMID: 12618959 2. Bennati, AM. et al. (2006) Biochimica et biophysica acta 1761, 677-85. PMID: 16784888 3. Clayton, P. et al. (2010) Nucleus (Austin, Tex.) 1, 354-66. PMID: 21327084 4. Tsai, PL. et al. (2016) eLife 5. PMID: 27336722 5. Silve, S. et al. (1998) Biochimica et biophysica acta 1392, 233-44. PMID: 9630650 6. Duband-Goulet, I. et al. (2000) Biochemistry 39, 6483-8. PMID: 10828963
Note	For research use only.

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SDS-PAGE for Recombinant Human LBR Protein, N-GST & C-His

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Datasheet

Human LBR Recombinant Protein (N-GST & C-His).pdf

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Human LBR Recombinant Protein (N-GST & C-His) (HC667012)

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