Human LIPC Recombinant Protein (N-His) (HY234012)

Name: Human LIPC Recombinant Protein (N-His)
Brand: abinScience
SKU: HY234012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY234012
Description	Recombinant Human LIPC Protein, N-His (HY234012) expressed in E. coli, spanning Glu47-Pro478. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P11150
Protein length	Glu47-Pro478
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	50.6 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Phospholipase A1, Lipase member C, LIPC, Hepatic lipase, Hepatic triacylglycerol lipase, Lysophospholipase, HTGL, HL
Background	Hepatic triacylglycerol lipase (LIPC) is a ~55 kDa protein. Catalyzes the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins, including chylomicrons, intermediate density lipoproteins (IDL), low density lipoproteins (LDL) of large size and high density lipoproteins (HDL), releasing free fatty acids (FFA) and smaller lipoprotein particles. Also exhibits lysophospholipase activity. Can hydrolyze both neutral lipid and phospholipid substrates but shows a greater binding affinity for neutral lipid substrates than phospholipid substrates. In native LDL, preferentially hydrolyzes the phosphatidylcholine species containing polyunsaturated fatty acids at sn-2 position. 1. McCoy, MG. et al. (2002) Journal of lipid research 43, 921-9. PMID: 12032167 2. Yang, P. et al. (2015) Biochimica et biophysica acta 1851, 1327-36. PMID: 26193433 3. Dugi, KA. et al. (1995) The Journal of biological chemistry 270, 25396-401. PMID: 7592706 4. Hill, JS. et al. (1996) The Journal of biological chemistry 271, 22931-6. PMID: 8798474
Note	For research use only.

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SDS-PAGE for Recombinant Human LIPC Protein, N-His

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Datasheet

Human LIPC Recombinant Protein (N-His).pdf

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Human LIPC Recombinant Protein (N-His) (HY234012)

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